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Single Cell

Sequencing

Breakthroughs in the coming decades will transform the world. We accelerate this progress by powering fundamental research across the life sciences, including oncology, immunology, and neuroscience.

About

The analysis of individual cells allows researchers to gain new knowledge in a diverse set of research areas, including developmental biology, tumor heterogeneity and pathogenesis and disease progression. It allows the comparison of genomic / transcriptomes / epigenome of individual cells and allows the evaluation of similarities and differences within a population of cells. The 10x Genomics' Chromium Single Cell system using Diploide is the single cell profiling platform that allows the analysis of large numbers of cells with the highest capture efficiency (up to 65%). Its technology divides the reactions into nanoliter scale drops containing pearls with unique barcodes called GEM (Gel Bead-In EMulsions). This core technology can be used to divide individual cells or high molecular weight DNA to prepare next-generation sequencing libraries in parallel.

The single cell service using the Diploid Chromium 10xG platform allows for reliable investigation of the genomic, transcriptomic and epigenomic individual cells with minimal bias. We can offer complete solutions for:

 

  • Single cell genomics (variation of copy number).

  • Transcriptomic of individual cells (gene expression profile and immune profile of T / B cells).

  • Epigenomics of individual cells (chromatin accessibility, ATAC-seq of individual cells).

How does it work.

Prepare the sample

Mail it to our lab

Postage is prepaid

Benefits

  • Up to 80,000 cells analyzed per chip with up to 65% cell capture efficiency

  • Highest throughput sequencing platforms, including the Illumina NovaSeq 6000, provide cost-effective single-cell solutions

  • Samples accepted at different stages of the 10x workflow - cells, Gel Beads in Emulsion (GEMs), and 10x barcoded cDNA libraries

  • *NEW* Dead cell removal improves data quality, especially for projects with suboptimal samples.

  • Proprietary cell freezing protocol maintains cell viability during transit and provides a convenient method to ship samples

  • Interactive analysis report provides an intuitive way to explore the data and find biological insights

Platform

SINGLE CELL GENE EXPRESSION
SINGLE CELL ATAC
SINGLE CELL INMUNE PROFILING
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© 2020 by DIPLOIDE GENETICS CORPORATE

1440 G St NW Washington, DC 20005

+1 833 DIPLOID

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